期刊
MICROCHIMICA ACTA
卷 186, 期 9, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3767-0
关键词
Fluorescence; DNAzyme; Entropy-driven amplification; Structure switching
资金
- Open Project of State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials [17kffk06]
- Project of Wenzhou Science & Technology Bureau [W20170006]
- National Natural Science Foundation of China [31300819]
- Sichuan Science and Technology Program [2018GZ0521]
- China Postdoctoral Science Foundation [2019 M653475]
An ultra-sensitive and turn-on method is demonstrated for the determination of uranyl ion. The assay is based on hairpin-to-DNAzyme structure switching that is induced by an entropy-driven catalytic reaction. An UO22+-specific DNAzyme is cleaved by UO22+ to produce a DNA fragment. This fragment initiates the entropy-driven catalytic reaction to produce a large number of a sequence R. The sequence R initiates the circular cleavage of FAM-labeled hairpins by switching the hairpin to Mg2+-specific DNAzyme structure. This causes the recovery of green fluorescence. The method works in the 20 pM to 800 pM concentration range and the limit of detection is 4 pM. Graphical abstract Entropy driven catalytic reaction induced hairpin structure switching for fluorometric detection of uranyl ions.
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