期刊
MICROCHIMICA ACTA
卷 186, 期 9, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3727-8
关键词
Dual-signal probe; Colorimetry; Fluorometry; Turn-on; Urine samples
资金
- National Natural Science Foundation of China [81701766, 81702998]
A colorimetric/fluorometric dual-signal assay is described for the determination of dopamine (DA). A nanoprobe was obtained by linking a 4-(4-dialkylaminostyryl)pyridinium derived fluorophore to citrate-capped gold nanoparticles (AuNPs). The fluorescence of the fluorophore is quenched by the AuNPs via fluorescence resonance energy transfe. In the presence of DA, the catechol group of DA can absorb on the surface of AuNPs to induce aggregation, which is accompanied by a color change from red to blue. The yellow fluorescence of the fluorophore with excitation/emission maximum at 365/570 nm is recovered. The dual-signal detection allows the quantitative analysis of DA within 300 mu M by the colorimetric method and 80 mu M by the fluorometric method. The detection limits for the colorimetric/fluorometric methods are 1.85 mu M and 0.29 mu M, respectively. Quantitative determination of DA in spiked urine samples was successfully demonstrated, with recoveries ranging from 98.2 to 106.0%. Graphical abstract A colorimetric/fluorometric dual-signal assay is described for the determination of dopamine by linking a fluorophore to gold nanoparticles. The dopamine causes aggregation of the nanoparticles to induce color change, which is followed by the recovery of the fluorescence.
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