Effect of Head Structure on ATP Detection in Polydiacetylene Systems

ATP detection systems were formed using 10,12-pentacosadiynoic acid derivatives that had a tertiary amine group at their end. Ortho, meta, and para derivatives were synthesized and the receptiveness for ATP using these derivatives was characterized. A vesicle did not form with the ortho derivative. The para derivative, did form a vesicle but was unable to detect ATP. Only the meta derivative showed a color change from blue to red upon ATP detection. The size of the vesicle using a meta derivative was larger than that formed when using a para derivative. The meta derivative did not respond with a color change to analogs of ATP. The concentration of ATP that was able to be detected by the naked eye was 100 mu M, and that by the UV spectrum was 50 mu M. An ATP detection system was developed using polydiacetylene systems in this study.