4.5 Article

Circulating tumor cell clusters are a potential biomarker for detection of non-small cell lung cancer

期刊

LUNG CANCER
卷 134, 期 -, 页码 147-150

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ELSEVIER IRELAND LTD
DOI: 10.1016/j.lungcan.2019.06.016

关键词

Circulating tumor cells; Liquid biomarkers; Non-small cell lung cancer; Lung cancer screening

资金

  1. Mizzou Advantage Interdisciplinary Research Grant (MAIRG)

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Objectives: Circulating tumor cell (CTC) clusters (>= 2 CTCs in aggregate) detected in the peripheral blood have predictive value in solid cancers, including non-small cell lung cancer (NSCLC). The goal of the study was to investigate the presence of CTC clusters in NSCLC patients and in high-risk screening subjects having no or benign nodules in a screening low-dose CT (LDCT). Materials and methods: In a prospective pilot trial, 7.5 ml peripheral blood was collected from treatment-naive NSCLC patients, LDCT screening subjects (55-80 years, >= 30 pack-year smoking history) with no (Lung-RADS 1) or benign lung nodules (Lung-RADS 2), and healthy never-smoking controls. CTCs were enriched by size, also allowing CTC cluster isolation. For CTC identification and enumeration, immunofluorescence staining was performed for cytokeratins (CK) 8/18 and/or 19, EpCAM, CD45, and nuclei were stained with DAPI. Clinicopathological data were collected, and LDCT interpreted by the American College of Radiology Lung-RADS criteria. Results: CTC clusters were detected in 12/29 (41.4%) of all NSCLC patients, but not found in 31 high-risk screening subjects with Lung-RADS 1 or Lung-RADS 2 (P < 0.05). Since non-clustered, single CTCs were detectable in both groups of NSCLC patients (100%) and in 18/31 (58.1 %) of high-risk screening subjects. No CTCs were detected in 20 healthy control subjects. Conclusion: This pilot study suggests that CTC clusters are a useful and specific liquid biomarker to further explore for screening by LDCT and risk stratification of NSCLC patients. Future prospective studies with higher subject numbers will need to be performed.

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