期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 141, 期 36, 页码 14043-14047出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.9b05894
关键词
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资金
- National Natural Science Foundation of China [21677170, 21876194]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB14010300]
Simultaneously monitoring label-free nanoparticles (NPs) and fluorescent biomolecules inside the live cell in real time is challenging because both imaging methods require different instrumentation and measuring principles. Here we report a novel scattered light imaging (SLi) technique that allows label-free NPs to be monitored using a conventional confocal microscope. The method shows a high spatial resolution and can distinguish label-free silver nanoparticles (AgNPs) with a 10 nm size difference in live cells. We performed SLi to observe the uptake, movement, distribution, and transformation of AgNPs in live cells at a single-particle level. The method is applicable to accurately track the localization of a variety of nanomaterials inside the cell. With this approach, label-free NP and fluorescent-labeled biomolecules are imaged simultaneously making it possible to real-time monitor nanobio interactions.
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