期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 141, 期 37, 页码 14699-14706出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.9b06237
关键词
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资金
- Cancer Prevention Research Institute of Texas (CPRIT) [RR170014]
- Robert A. Welch Foundation [C-1970, Q-0035, BE-1913]
- Hamill Innovation Award (Hamill Foundation)
- John S. Dunn Foundation Collaborative Research Award (Gulf Coast Consortia)
- NIH [R35-GM133706, F32-EY027171, R01-GM112003, R01-EY026545]
Photoactivatable fluorophores afford powerful molecular tools to improve the spatial and temporal resolution of subcellular structures and dynamics. By performing a single sulfur-for-oxygen atom replacement within common fluorophores, we have developed a facile and general strategy to obtain photoactivatable fluorogenic dyes across a broad spectral range. Thiocarbonyl substitution within fluorophores results in significant loss of fluorescence via a photoinduced electron transfer-quenching mechanism as suggested by theoretical calculations. Significantly, upon exposure to air and visible light residing in their absorption regime (365-630 nm), thio-caged fluorophores can be efficiently desulfurized to their oxo derivatives, thus restoring strong emission of the fluorophores. The effective photoactivation makes thio-caged fluorophores promising candidates for super-resolution imaging, which was realized by photoactivated localization microscopy (PALM) with low-power activation light under physiological conditions in the absence of cytotoxic additives (e.g., thiols, oxygen scavengers), a feature superior to traditional PALM probes. The versatility of this thio-caging strategy was further demonstrated by multicolor super-resolution imaging of lipid droplets and proteins of interest.
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