期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 141, 期 34, 页码 13454-13458出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.9b04659
关键词
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资金
- Strategic Priority Research Program of Chinese Academy of Sciences [XDB29050201]
- National Natural Science Foundation of China [21675119, 21705110, 81772737]
- National Major Science and Technology Projects [2018ZX10301405]
- Natural Science Foundation of Guangdong [2018B030306046]
- China Postdoctoral Science Foundation [BX201700161, 2017M622860]
- Shenzhen Municipal Government of China [JCYJ20170413161749433, JSGG20160301161836370]
- Sanming Project of Shenzhen Health and Family Planning Commission [SZSM201412018, SZSM201512037]
- High Level University's Medical Discipline Construction [2016031638]
- Shenzhen Health and Family Planning Commission [201606019]
Detection and imaging RNAs in live cells is in high demand. Methodology for such a purpose is still a challenge, particularly for single RNA detection and imaging in live cells. In this study, a type of quantum dot (QD) nanobeacon with controllable valencies was constructed by precisely conjugating the black hole quencher (BHQ1) and phosphorothioate comodified DNA onto CdTe:Zn2+ QDs via a one-pot hydrothermal method. The nanobeacon with only one conjugated DNA was used to label and detect low-abundance nucleic acids in live cells, and single HIV-1 RNAs were detected and imaged in live HIV-1 integrated cells. Additionally, QD nanobeacon-labeled HIV-1 genomic RNAs were encapsulated in progeny viral particles, which can be used to track the uncoating process of single viruses. The current study provides a platform for nucleic acid labeling and imaging with high sensitivity, being especially meaningful for tracking of individual RNAs in live cells.
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