期刊
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
卷 19, 期 9, 页码 5441-5447出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2019.16547
关键词
Gold Nanoparticles; Cytotoxicity; MTT and TUNEL Assay; Neural Progenitor Cell
类别
资金
- Original Technology Research Program for Brain Science through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2006-2005378, 2009-0093910]
- National Research Foundation of Korea [2006-2005378] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Gold nanoparticles (GNPs) are utilized as diagnostic and therapeutic tools to detect and treat human disease. Researchers in the field of nanotoxicity are working to determine the physicochemical properties of nanoparticles that lead to toxicity in an effort to establish safe design rules. In this study, we performed the MTT and terminal transferase dUTP nick end labeling (TUNEL) assays to verify the cytotoxicity of GNPs on rat brain tissue and human neural progenitor cells (NPCs). As results, we observed that GNPs induced apoptosis in NPCs. NPCs were markedly damaged following the administration of 200 mu M and 2 mM GNPs, whereas 2 mu M GNPs showed slightly increased damage relative to that of the control. In addition, TUNEL-positive cells were densely distributed at regions surrounding the GNP injection site in the brain 7 days after the GNPs injection. During long-term GNPs exposure, TUNEL-positive cells were rarely observed in the cerebral cortex. In this study, we observed that apoptosis increased in proportion to GNP concentrations in the brain and in cultivated NPCs. These result suggest that large GNPs (<100 nm) are toxic and that the cytotoxicity increased as the concentration of GNPs increased in NPCs or in the brain.
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