期刊
JOURNAL OF MEDICAL MICROBIOLOGY
卷 68, 期 11, 页码 1591-1595出版社
MICROBIOLOGY SOC
DOI: 10.1099/jmm.0.001085
关键词
Pseudomonas aeruginosa environmental antibiotic resistance genetic variant cystic fibrosis
类别
资金
- Health Research Council of New Zealand [17/372]
- University of Otago Doctoral Scholarship
- Australian National Health and Medical Research Council Project [455919]
- Prince Charles Hospital Foundation
- Queensland Health HRF
- Wellington Medical Research Foundation
- Maurice and Phyllis Paykel Trust
Patient-derived isolates of the opportunistic pathogen Pseudomonas aeruginosa are frequently resistant to antibiotics due to the presence of sequence variants in resistance-associated genes. However, the frequency of antibiotic resistance and of resistance-associated sequence variants in environmental isolates of P. aeruginosa has not been well studied. Antimicrobial susceptibility testing (ciprofloxacin, ceftazidime, meropenem, tobramycin) of environmental (n=50) and cystic fibrosis (n=42) P. aeruginosa isolates was carried out. Following whole genome sequencing of all isolates, 25 resistance-associated genes were analysed for the presence of likely function-altering sequence variants. Environmental isolates were susceptible to all antibiotics with one exception, whereas patient-derived isolates had significant frequencies of resistance to each antibiotic and a greater number of likely resistance-associated genetic variants. These findings indicate that the natural environment does not act as a reservoir of antibiotic-resistant P. aeruginosa, supporting a model in which antibiotic susceptible environmental bacteria infect patients and develop resistance during infection.
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