期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 377, 期 -, 页码 299-304出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2019.05.108
关键词
Bacterial endospores; Dipicolinic acid; BODIPY; Copper complex; Ratiometric detection
资金
- Konya Food and Agriculture University (KFAU)
- Nanobiz RD Ltd.
A new catechol-substituted monostyryl boradiazaindacene (BODIPY)-based lanthanide-free colorimetric and fluorogenic probe was developed for the sequential detection of Cu2+ ions and dipicolinic acid (DPA), a distinctive biomarker of bacterial endospores, with high sensitivity and selectivity. In the presence of Cu2+ ions, the blue solution of the probe changes to cyan and the fluorescence is quenched, however, the cyan color changes to blue immediately and the fluorescence is restored on contact with DPA, resulting from competitive binding of DPA that interact with Cu2+ ions. A practical application by using Geobacillus stearothermophilus spores was further studied and as low as 1.0 x 10(5) spores were detected.
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