期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 849, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2019.113381
关键词
Magnetic nanoparticles; Glucose oxidase; Sandwich immunoreaction; Disposable electrode; Therapeutic drug monitoring
资金
- Advanced Connection Technology Inc.
- Ministry of Science and Technology, Taiwan [MOST107-2313-B-005-006-MY3, MOST107-2321-B-005-014]
- Innovation and Development Center of Sustainable Agriculture from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education, Taiwan (MOE)
- National Science Foundation
- [TCVGH-NCHU1057605]
Adalimumab (Ada) is widely used to treat autoimmune diseases, and a correct dose of Ada can maintain therapeutic effectiveness and relieve symptoms. This study develops a rapid quantifying method of Ada using magnetic nanoparticle (MNP)-based immunoreaction and disposable screen-printed carbon electrodes (SPCEs). Anti-Ada Fab fragment and mouse anti-human antibody is respectively used as primary antibody (Ab1) and secondary antibody (Ab2). Glucose oxidase (GOD) and the Ab2 were co-immobilized on silica nanoparticles (SiNPs) with the concentration ratio of 3:1 to promise good immune affinity and the more amount of immobilized GOD. After sequentially immunoreacting Ada with Ab1@MNPs and GOD-Ab2@SiNPs, the separated GOD-Ab2@SiNPs/Ada/Ab1@MNPs complex was dripped on the glucose/ferricyanide-coated SPCEs for GOD catalysis, and then the anodic current of reduced ferrocyanide was used for the quantification of Ada. The MNP-based immunoassays have good linearity from 0.1 mu g/mL to 2 mu g/mL and a limit of detection of 15.5 ng/mL Moreover, the immunoassays can practically measure the Ada concentrations in 10-fold diluted patient serum samples with good recovery. The operation time of MNP-based immunoassays takes only about 0.5 h. The disposable MNP-based immunoassays have great promise to develop a rapid quantification platform for therapeutic drug monitoring. (C) 2019 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据