期刊
JOURNAL OF DENTAL RESEARCH
卷 98, 期 11, 页码 1262-1270出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0022034519871021
关键词
dental cementum; periodontal ligament; Wnt signaling pathway; cell lineage; transgenic mice; 3-dimensional imaging
资金
- National Natural Science Foundation of China [81700980]
- Sichuan Province Science and Technology Support Program [2019YJ0097]
- Postdoctoral Research Foundation of Sichuan University [2018SCU12018]
- U.S. National Institutes of Health [DE025014, DE025659]
To date, attempts to regenerate functional periodontal tissues (including cementum) are largely unsuccessful due to a lack of full understanding about the cellular origin (epithelial or mesenchymal cells) essential for root cementum growth. To address this issue, we first identified a rapid cementum growth window from the ages of postnatal day 28 (P28) to P56. Next, we showed that expression patterns of Axin2 and beta-catenin within cementum-forming periodontal ligament (PDL) cells are negatively associated with rapid cementum growth. Furthermore, cell lineage tracing studies revealed that the Axin2(+)-mesenchymal PDL cells and their progeny rapidly expand and directly contribute to postnatal acellular and cellular cementum growth. In contrast, the number of K14(+) epithelial cells, which were initially active at early stages of development, was reduced during rapid cementum formation from P28 to P56. The in vivo cell ablation of these Axin2(+) cells using Axin2(CreERT2/+); R26R(DTA/+) mice led to severe cementum hypoplasia, whereas constitutive activation of beta-catenin in the Axin2(+) cells resulted in an acceleration in cellular cementogenesis plus a transition from acellular cementum to cellular cementum. Thus, we conclude that Axin2(+)-mesenchymal PDL cells, instead of K14(+) epithelial cells, significantly contribute to rapid cementum growth.
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