Cladophora fascicularisMediated Silver Nanoparticles: Assessment of Their Antibacterial Activity AgainstAeromonas hydrophila

This study reports on the bioactive potential of silver nanoparticle synthesized from seaweed,Cladophora fascicularisaqueous extract.C. fascicularisassisted silver nanoparticles (Cf-AgNPs) was characterized by UV-visible spectroscopy, XRD, FT-IR, FESEM, TEM, and EDX analysis. The Cf-AgNPs exhibited a maximum absorption peak at 427 nm. The carboxyl (-C=O) and amine (N-H) groups ofC. fascicularisextract is answerable for the synthesis of Cf-AgNPs. The microscopic analysis confirmed that the synthesized Cf-AgNPs are spherical in shape with an average size of 45 nm. The Cf-AgNPs exhibited a dose depended antibacterial activity against Aeromonas hydrophila. Noteworthy bacterial inhibition (19 mm) was observed at 150 mu g/ml, whereas, the lowest activity (10 mm) was recorded at 25 mu g/ml. Similarly, Cf-AgNPs significantly (P < 0.05) enhanced the protein leakage inA. hydrophilaby increasing the membrane permeability. The considerable amount of ROS activity was noticed in the test bacteriumA. hydrophilawhen treated with 150 mu g/ml of Cf-AgNPs. The toxicity investigation results revealed that the synthesized Cf-AgNPs were not acutely toxic toArtemianauplii even at a higher concentration (150 mu g/ml). These results underline that Cf-AgNPs effectively inhibited the growth ofA. hydrophilaand showed the non-toxic effect with outstanding biocompatibility.