期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1610, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.chroma.2019.460537
关键词
3D printing; Multi-channel device; Dopamine metabolism; Electrochemical detection; Polymer monoliths; Titanium
资金
- Czech Science Foundation [1711252S]
- STAMP project (Separation Technology for A Million Peaks) under the Horizon 2020 Excellent-Science program of the European Research Council (ERC) [694151]
A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device. (C) 2019 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据