期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1601, 期 -, 页码 274-287出版社
ELSEVIER
DOI: 10.1016/j.chroma.2019.06.009
关键词
Opuntia stricta var. dillenii; Cactaceae; Betalains; Flavonoid glycosides; Off-line ESI-MS/MS injection metabolite profiling; Analytical/semi-preparative ion-pair high-performance countercurrent chromatography; Electrospray mass-spectrometry based metabolite partition ratio K-D-determination
资金
- Vietnamese National Foundation for Science and Technology Development
Electrospray mass spectrometry-profiling guided the metabolome investigation of a C18 reversed phase adsorbate of Opuntia stricta var. dillenii fruits following analytical, and semi-preparative high-performance countercurrent chromatography (HPCCC) fractionation, and visualization of molecular weight elution profiles based on selected single ion-traces. Experimental partition-ratio values K-D, and peak widths for detected metabolites were determined. Structural characterization of metabolites, and co-elution effects were monitored in the scan range m/z 150-2200. The polar ion-pair activated solvent system tert.-butylmethylether - n-butanol - acetonitrile - water (0.7% trifluoroacetic acid) [2:2:1:5]) was used for partition-ratio K-D improvement of ionic betalains. HPCCC operations were in the head-to-tail mode using the elution-extrusion approach. Selected ESI-MS ion traces visualized the elution of fourty-three metabolites, whereas twenty-one were identified as known betalain pigments, and their classic degradation products, and chlorinated betacyanin artefacts. Potentially, novel fruit metabolites of Opuntia were recognized by unknown molecular weights, and MS/MS fragmentations. Off-line ESI-MS fraction monitoring determined peak elution windows, and resulted in K-D-based chromatographic scales. Detectable metabolites were compared by separation-alpha, and resolution factors Rs revealing a better performance of the analytical HPCCC experiment. Experimental metabolite K-D-values from analytical and semi-preparative HPCCC runs were widely consistent, and confirmed the reproducibility of the technique based on the used sample material. (C) 2019 Published by Elsevier B.V.
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