期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1606, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.chroma.2019.460425
关键词
Nano-LC; Capillary electrochromatography; Enantioseparations; Covalently immobilized polysaccharide-based chiral stationary phase; Amylose tris(3-chloro-5-methylphenylcarbamate)
In the present study separation of enantiomers of some chiral neutral and weakly acidic analytes was investigated on the chiral stationary phase (CSP) made by covalent immobilization of amylose tris(3-chloro-5-methylphenylcarbamate) onto silica in nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC) in acetonitrile and aqueous acetonitrile. Few comparisons were made also between the enantioseparations in nano-LC and high-performance liquid chromatography (HPLC) with the chiral column of 4.6 x 250 mm dimension. Slightly better separation of enantiomers was observed in HPLC mode compared to nano-LC mode. It was shown that in the capillary columns packed with the CSP containing about 20% (w/w) of a covalently immobilized neutral chiral selector, amylose tris(3-chloro-5-methylphenylcarbamate), sufficient electroosmotic flow has been generated and enantioseparations with reasonable analysis time were performed also in CEC mode. It was shown once again that CEC offers a clear advantage over nano-LC from the viewpoint of plate numbers and peak resolution. (C) 2019 Elsevier B.V. All rights reserved.
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