期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 235, 期 3, 页码 3096-3111出版社
WILEY
DOI: 10.1002/jcp.29215
关键词
collagen; matrix-signaling; focal adhesion kinase
资金
- Canadian Institutes of Health Research Operating Grant [MOP-416228]
- Canada Research Chair in Matrix Dynamics
Focal adhesion kinase (FAK) is critical for collagen expression but its regulation of collagen remodeling is not defined. We examined the role of FAK in the degradation and reorganization of fibrillar collagen. Compared with wild-type (WT) mouse embryonic fibroblasts, FAK null (FAK(-/-)) fibroblasts generated twofold (p < .0001) higher levels of 3/4 collagen I fragment and expressed up to fivefold more membrane-type matrix metalloproteinase (MMP). When plated on stiff collagen substrates, compared with WT, FAK(-/-) cells were smaller (threefold reduced cell surface area; p < .0001) and produced fivefold fewer cell extensions (p < .0001) that were 40% shorter (p < .001). When cultured on soft collagen gels (stiffness of similar to 100 Pa) for 6-48 hr, cell spreading and cell extension formation were reduced by greater than twofold (p < .05 and p < .0001, respectively) while collagen compaction and alignment were reduced by approximately 30% (p < .0001) in FAK(-/-) cells. Similar results were found after treatment with PF573228, a FAK inhibitor. Reconstitution of FAK(-/-) cells with FAK mutants showed that compared with WT, cell extension formation was reduced twofold (p < .0001) in the absence of the kinase domain and sixfold (p < .0001) with a Y397F mutant. Enhanced collagen degradation was exhibited by the mutants (similar to threefold increase; p < .0001 of 3/4 collagen fragments without kinase domain or Y397F mutant; p < .01). Compared with FAK(+/+) cells, matrices produced by FAK(-/-) cells generated higher levels of beta(1) integrin activation (p < 0.05), extracellular-signal-regulated kinase (ERK) phosphorylation, and production of 3/4 collagen I fragment by human gingival fibroblasts. Collectively these data indicate that (a) the kinase activity of FAK enhances collagen remodeling by tractional forces but inhibits collagen degradation by MMPs; (b) FAK influences the biological activity of fibroblast-secreted extracellular matrices, which in turn impacts beta 1 integrin and ERK signaling, and collagen degradation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据