期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 235, 期 3, 页码 3033-3042出版社
WILEY
DOI: 10.1002/jcp.29208
关键词
binding motif; ChIP-qPCR; promoter; STRA8; transcription suppressor
资金
- National Natural Science Foundation of China [81200472/H0425]
- Fundamental Research Funds for the Central Universities in China [KYTZ201602]
- National Innovation and Entrepreneurship Training Program for Undergraduates [201710307052]
Promyelocytic leukaemia zinc finger (PLZF) is a key factor in inhibiting differentiation of spermatogonial progenitor cells (SPCs), but the underlying mechanisms are still largely unknown. In this study, the regulation of PLZF on Kit, Stra8, Sohlh2, and Dmrt1 (SPCs differentiation related genes) was investigated. We found some PLZF potential binding sites existed in the promoters of Kit, Stra8, Sohlh2, and Dmrt1. Additionally, the expressions of KIT, STRA8, SOHLH2, and DMRT1 were upregulated when PLZF was knockdown in SPCs. Furthermore, chromatin immunoprecipitation quantitative polymerase chain reaction revealed PLZF directly bound to the promoters of Kit, Stra8, Sohlh2, and Dmrt1. Besides, dual luciferase assay verified PLZF repressed those gene expressions. Collectively, our finding indicate that PLZF binds to the promoter regions of Kit, Stra8, Sohlh2, and Dmrt1 to regulate SPCs differentiation, which facilitate us to further understand the regulatory mechanism of PLZF in SPCs fates.
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