期刊
JOURNAL OF CELL SCIENCE
卷 132, 期 17, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.225383
关键词
Cardiac potassium channel; Endocytosis; Focal adhesion; Mechanotransduction; Micropatterning
类别
资金
- National Institutes of Health [1R01HL126524, 1R01HL132389, U01HL134764]
- National Science Foundation
How ion channels localize and distribute on the cell membrane remains incompletely understood. We show that interventions that vary cell adhesion proteins and cell size also affect the membrane current density of inward-rectifier K+ channels (K(ir)2.1; encoded by KCNJ2) and profoundly alter the action potential shape of excitable cells. By using micropatterning to manipulate the localization and size of focal adhesions (FAs) in single HEK293 cells engineered to stably express K(ir)2.1 channels or in neonatal rat cardiomyocytes, we establish a robust linear correlation between FA coverage and the amplitude of K(ir)2.1 current at both the local and whole-cell levels. Confocal microscopy showed that Ki r 2.1 channels accumulate in membrane proximal to FAs. Selective pharmacological inhibition of key mediators of protein trafficking and the spatially dependent alterations in the dynamics of K(ir)2.1 fluorescent recovery after photobleaching revealed that the K(ir)2.1 channels are transported to the cell membrane uniformly, but are preferentially internalized by endocytosis at sites that are distal from FAs. Based on these results, we propose adhesion-regulated membrane localization of ion channels as a fundamental mechanism of controlling cellular electrophysiology via mechanochemical signals, independent of the direct ion channel mechanogating.
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