期刊
JOURNAL OF CELL BIOLOGY
卷 218, 期 10, 页码 3397-3414出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201807106
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资金
- Natural Sciences and Engineering Research Council of Canada
- Yoshio Masui Prize in Developmental, Molecular, and Cellular Biology
- Human Frontier Science Program
- Damon Runyon Cancer Research Foundation
- National Institutes of Health/National Institute of General Medical Sciences [R01-GM084947, R01-GM067761]
- Japan Society for the Promotion of Science KAKENHI [JP17H03658]
- Canadian Institutes of Health Research
The spatio-temporal regulation of small Rho GTPases is crucial for the dynamic stability of epithelial tissues. However, how RhoGTPase activity is controlled during development remains largely unknown. To explore the regulation of Rho GTPases in vivo, we analyzed the Rho GTPase guanine nucleotide exchange factor (RhoGEF) Cysts, the Drosophila orthologue of mammalian p114RhoGEF, GEF-H1, p190RhoGEF, and AKAP-13. Loss of Cysts causes a phenotype that closely resembles the mutant phenotype of the apical polarity regulator Crumbs. This phenotype can be suppressed by the loss of basolateral polarity proteins, suggesting that Cysts is an integral component of the apical polarity protein network. We demonstrate that Cysts is recruited to the apico-lateral membrane through interactions with the Crumbs complex and Bazooka/Par3. Cysts activates Rho1 at adherens junctions and stabilizes junctional myosin. Junctional myosin depletion is similar in Cysts- and Crumbs-compromised embryos. Together, our findings indicate that Cysts is a downstream effector of the Crumbs complex and links apical polarity proteins to Rho1 and myosin activation at adherens junctions, supporting junctional integrity and epithelial polarity.
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