期刊
JOURNAL OF CELL BIOLOGY
卷 218, 期 9, 页码 3098-3116出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201903124
关键词
-
类别
资金
- National Institutes of Health Office of Research Infrastructure Programs [P40 OD010440]
- American Cancer Society postdoctoral fellowship [129351-PF-16-024-01-CSM]
- National Institute of General Medical Sciences (Maximizing Investor's Research Award) [R35 GM118049]
- National Institute of Child Health and Human Development [R21 HD084290]
Basement membranes (BMs) are cell-associated extracellular matrices that support tissue integrity, signaling, and barrier properties. Type IV collagen is critical for BM function, yet how it is directed into BMs in vivo is unclear. Through live-cell imaging of endogenous localization, conditional knockdown, and misexpression experiments, we uncovered distinct mechanisms of integrin-mediated collagen recruitment to Caenorhabditis elegans postembryonic gonadal and pharyngeal BMs. The putative laminin-binding alpha INA-1/beta PAT-3 integrin was selectively activated in the gonad and recruited laminin, which directed moderate collagen incorporation. In contrast, the putative Arg-Gly-Asp (RGD)-binding alpha PAT-2/PPAT-3 integrin was activated in the pharynx and recruited high levels of collagen in an apparently laminin-independent manner. Through an RNAi screen, we further identified the small GTPase RAP-3 (Rap1) as a pharyngeal-specific PAT-2/beta PAT-3 activator that modulates collagen levels. Together, these studies demonstrate that tissues can use distinct mechanisms to direct collagen incorporation into BMs to precisely control collagen levels and construct diverse BMs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据