期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 38, 页码 10744-10755出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b04488
关键词
beta-glucosidase; transglycosylation; hydrolysis; Coprinopsis cinerea; 3-O-beta-D-gentiobiosyl-D-laminarioligosaccharides
资金
- National Natural Science Foundation of China [31570046, 31600053]
- Priority Academic Development Program of Jiangsu Higher Education Institutions
We previously reported that beta-glucosidase BGL1 at low concentration (15 mu g mL(-1)) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose. This study shows that BGL1 at high concentration (200 mu g mL(-1)) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively. Interestingly, BGL1 at low concentration (1.5 mu g mL(-1)) showed transglycosylation but BGL1 at high concentration (200 mu g mL(-1)) did not. BGL1 utilizes only laminarioligosaccharides but not glucose, gentiobiose, and cellobiose to synthesize the higher oligosaccharides. BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a beta(1 -> 3) or beta(1 -> 6) fashion to produce higher laminarioligosaccharides or 3-O-beta-D-gentiobiosyl-D-laminarioligosaccharides. The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.
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