期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 38, 页码 10563-10576出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b02404
关键词
2-cysteine peroxiredoxin (2-CP); caffeic acid O-methyltransferase (COMT); cadmium; cellular redox homeostasis; melatonin; phytochelatins; sulfur metabolism; tomato
资金
- National Key Research and Development Program of China [2016YFD0201001]
- National Natural Science Foundation of China [31950410555, 31850410469]
- Zhejiang Provincial Natural Science Foundation of China [LY18C150001]
- Henan University of Science and Technology (HAUST) Research Start-up Fund for New Faculty [13480058]
- China Postdoctoral Science Foundation [2016T90544, 2015M580515]
Sulfur (5) metabolism plays a vital role in Cd detoxification, but the collaboration between melatonin biosynthesis and S metabolism under Cd stress remains unaddressed. Using exogenous melatonin, melatonin-deficient tomato plants with a silenced caffeic acid O-methyltransferase (COMT) gene, and COMT-overexpressing plants with cosuppression of sulfate transporter (SUT)1 and SUT2 genes, we found that melatonin deficiency decreased S accumulation and aggravated Cd phytotoxicity, whereas exogenous melatonin or overexpression of COMT increased S uptake and assimilation, resulting in an improved plant growth and Cd tolerance. Melatonin deficiency promoted Cd translocation from root to shoot, but COMT overexpression caused the opposite effect. COMT overexpression failed to compensate the functional hierarchy of S when its uptake was inhibited by cosilencing of transporter SUT1 and SUT2. Our study provides genetic evidence that melatonin-mediated tolerance to Cd is closely associated with the efficient regulation of S metabolism, redox homeostasis, and Cd translocation in tomato plants.
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