期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 137, 期 -, 页码 657-665出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.07.012
关键词
Cheongkyool; Polysaccharide; MAPK; NF-kappa B; Rhamnogalacwronan-I
资金
- High Value-added Technology Development Program [316051-03-3-CG000]
- Ministry for Food, Agriculture, Forestry, and Fisheries, Republic of Korea (iPET)
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2018R1D1A1B07050646]
- Main Research Program of the Korea Food Research Institute (KFRI) - Ministry of Science and ICT [E0170602-03]
To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration. CCE-I highly enhanced the production of IL-6, TNF-alpha, and NO in RAW 264.7 cell lines. It augmented the mRNA expression of IL-6, TNF-alpha, and iNOS in a dose-dependent manner. Moreover, CCE-I dose-dependently induced phosphorylation of MAPK5 and NF-kappa B related proteins and led to the nuclear translocation of p65. The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors. Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after beta-elimination. The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains. (C) 2019 Elsevier B.V. All rights reserved.
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