期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 141, 期 -, 页码 1258-1270出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.09.048
关键词
Polyelectrolyte complex; Magnetic nanoparticles; Alginate; Bovine serum albumin; Curcumin
资金
- Institute for Advanced Studies in Basic Sciences (IASBS)
Curcumin-loaded hybrid magnetic nanoparticles (HMNPs) were formulated using a simple and green method. At first, surface of Fe3O4 nanoparticles was modified using alginate (ALG), and magnetic ALG/Fe3O4 nanoparticles with a size of 33 nm and a zeta potential of -25 my were prepared. Then, a complex including bovine serum albumin (BSA) and poly((3-acrylamidopropyl)trimethylammonium chloride) (p (APTMACl)) was prepared and optimized. The behavior of the BSA/p(APTMACl) complex was evaluated as a function of pH and protein/polymer ratio by UV-Vis spectroscopy, zeta potential, and DLS analysis. Finally, the hybrid complex of BSA/p(APTMACl)@ALG/Fe3O4 were prepared using mixing of the BSA/p (APTMACl) and ALG/Fe3O4 systems with opposite charges via electrostatic interactions. The prepared BSA/p(APTMACl) @ALG/Fe3O4 complex was named hybrid magnetic nanoparticles (HMNPs) and characterized by UV-Vis spectroscopy, SEM, FT-IR, TGA, XRD, VSM, and AFM. Three samples including pure Fe3O4, ALG/Fe3O4, and the final HMNPs exhibited no cytotoxic effect on MCF-7 breast cancer cell using the MTT assay. MTT assay to test the antitumor activity of bare and encapsulated-curcumin against MCF-7 cells showed BSA/p(APTMACl)@ALG/Fe3O4 nanoparticles are able to stabilize the curcumin anticancer drug and also enhancing the antitumor activity of the encapsulated curcumin. After loading of curcumin with a high efficiency of 95%. The accumulative release profiles of curcumin were investigated at 37 degrees C in phosphate buffered saline (PBS, pH 3 and 7.4). The release of curcumin-loaded HMNPs followed the Korsmeyer-Peppas equation and anomalous (non-fickian) diffusion mechanism at pHs 3 and 7.4. (C) 2019 Elsevier B.V. All rights reserved.
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