期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 137, 期 -, 页码 1050-1059出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.07.050
关键词
Bacterial cellulose; Chitosan; 3D scaffolds; Biocompatibility; Cancer diagnosis
资金
- National Natural Science Foundation of China [21774039, 51603079, 31270150]
- China Postdoctoral Science Foundation [2016M602291]
- Higher Education Commission of Pakistan (HEC) [21-1680/SRGP/R D/HEC/2017]
- Fundamental Research Funds for Central Universities, Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences
Bacterial cellulose (BC) has emerged as a biomaterial for diverse biomedical applications owing to its unique structural, physico-chemical, mechanical, and biological features. Its porous geometry and three-dimensional fibrous structure allow the impregnation of various materials into its matrix. The current study was aimed to fabricate 3D scaffolds of bacterial cellulose and chitosan (BC-Chi) through a one-step ex situ solution impregnation strategy and analyze the scaffold interaction with the ovarian cancer cell lines (A2780). Field emission scanning electron microscopy (FE-SEM) showed successful impregnation of chitosan into the BC matrix. Phase-contrast and confocal microscopy analyses revealed that human ovarian cancer cell lines (A2780) were adhered not only to the surface but deeply infiltrated into the matrix of BC-Chi scaffold. WST-1 assay, histology analysis, and cytoskeleton and nuclear staining showed high viability, proliferation, and infiltration of A2780 cell lines into the scaffold. The RT-PCR analysis revealed a decreased mRNA level of Notch receptors, indicating a strong cell-scaffold interaction. The improved biocompatibility, non-toxicity, and 3D structure of fabricated BC-Chi scaffold justify its potential applications diagnosis of ovarian cancer in vivo. (C) 2019 Elsevier B.V. All rights reserved.
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