期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 137, 期 -, 页码 433-441出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.06.246
关键词
Chitosan; Cellulose nanocrystals; Injectable thermo-sensitive hydrogel; Mesenchymal stem cells; Cutaneous wound
资金
- Lanri Biotechnology Co., Ltd.
- Natural Science Foundation of China, China [81573708, 31271019]
- Guangzhou Science and Technology Program key projects, China [201601010270, 2017010160489, 201704030083, 201907010032, 201907010037]
- Pearl River S&T Nova Program of Guangzhou, China [201710010155, 201806010072]
- Science and Technology Planning Project of Guangdong Province, China [2015A010101313, 2017A050506011, 2017A050501013, 2017B090911012, 2018A050506040, 2018A050506019, 2018A050506021]
Human umbilical cord-mesenchymal stem cells (hUCMSCs) can secrete a variety of cytokines and growth factors promoting wound repair. Hydrogel is suitable biomaterial to supply niche for cells adhesion and survival. This study constructed a functional injectable thermo-sensitive hydrogel (chitosan/glycerol phosphate sodium/cellulose nanocrystals, CS/GP/CNC) encapsulated hUCMSCs to repair full-thickness cutaneous wound. Addition of CNC to the CS/GP system not only accelerated the gel speed, but also greatly improved the mechanical properties of the gel and decreased degradation rate. The novel hydrogel was injectable and low toxicity. Histological detection showed that hydrogel-hUCMSCs combination significantly accelerated wound closure, microcirculation, tissue remodeling, re-epithelialization and hair follicle regeneration, and inhibited over-inflammation in the central and surrounding wounds. The hydrogel-hUCMSCs combination promoted collagen deposition and keratinocyte mature marker K1 expression, decreased inflammatory factors secretion namely TNF-alpha and IL-1 beta. The present data provides a potential strategy for treatment of non-healing chronic cutaneous wounds. (C) 2019 Elsevier B.V. All rights reserved.
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