期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 135, 期 -, 页码 998-1005出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.06.019
关键词
Exopolysaccharide; Structural characterization; Molecular morphology
资金
- National Key Research and Development Program [2018YFC0311203]
- Key Research and Development Program of Shandong [2018GHY115032]
Bacterial exopolysaccharides (EPSs) are widely applied in food, cosmetic, and medical industries. The EPS produced by Phyllobacterium sp. 921F was a novel polysaccharide, which exhibits attractive characteristics of high yield, favorable rheological properties, and excellent moisture retention ability. Considering the complexity of polysaccharide structures, specific enzymatic hydrolysis was employed here to resolve the structure of the EPS. End-products including tetra-, hexa- and octa-saccharides were isolated. According to their mass spectroscopy (MS) and nuclear magnetic resonance (NMR) spectra, the backbone of the EPS was found to be mainly comprising a -> 4)-beta-D-Glcp-(1 -> 3)-alpha-D-Galp(4,6-S-Pyr)-(1 -> disaccharide repeating units. Based on atomic force microscopy results, EPS exhibited characteristics that were consistent with a stiff, elongated molecule with no branches. The length and height of the single molecular chain were approximately 600 and 0.7 nm, respectively. Our clarification of structure and molecular morphology of EPS from Phyllobacterium sp. 921F provide a foundation for the industrial application of this potential moisture-retaining material. (C) 2019 Elsevier B.V. All rights reserved.
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