Preparation, characterization and evaluation of cellulose nanocrystal/poly(lactic acid) in situ nanocomposite scaffolds for tissue engineering

Cellulose nanocrystal (CNC)/poly(lactic acid) (PLA) in situ nanocomposite scaffolds were fabricated by in situ polymerization of lactic acid and CNC which was directly utilized as aqueous suspension, followed by a process of thermally induced phase separation. The CNC/PLA in situ nanocomposite porous scaffolds were characterized by mechanical test, protein adsorption, hemolysis test, in vitro degradation measurement, TEM, FTIR, SEM and WAXD. Compared to the PLA scaffold, the CNC/PLA in situ nanocomposite scaffolds showed a greatly increased compression modulus, an improved hemocompatibility and protein adsorption capacity. The inclusion of CNCs boosted the in vitro degradation of the in situ nanocomposite porous scaffolds and facilitated the deposition of Ca2+, CO32- PO43- ions in simulated body fluid. Furthermore, cell cultures were carried out on the CNC/PLA in situ nanocomposite porous scaffolds. In comparison with the PLA scaffold, the in situ nanocomposite scaffolds improved cell attachment and enhanced cell proliferation, denoting low cytotoxicity and good cytocompatibility. It can therefore be concluded that such scaffolds with excellent mechanical property, biocompatibility, biomineralization capacity and bioactivity hold great potential for bone tissue engineering. (C) 2019 Elsevier B.V. All rights reserved.