期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 134, 期 -, 页码 172-179出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2019.05.047
关键词
Sagittaria sagittifolia L.; Polysaccharides; SWE; Orthogonal experiment; Antioxidant activities
资金
- National Key R&D Program, China [2016YFD0400303]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province, China [KYCX17_1799]
In the present study, orthogonal experiment (L-9 (3)(4)) was used to optimize the extraction process of Sagittaria sagittifolia L. polysaccharides (SSP) with subcritical water, and the antioxidant activities of polysaccharides were also investigated. The results showed that the optimum extraction conditions were obtained when pH, extraction temperature, extraction time, and liquid to solid ratio were 7,170 degrees C, 16 min and 30:1 (mL/g), respectively. In addition, DPPH/ABTS radical scavenging rate and Fe3+ reducing power of polysaccharides exhibited a certain dose-effect relationship. Furthermore, both yield and antioxidant activities of polysaccharides with sub critical water extraction (SWE) were higher than traditional hot water extraction (HWE). The above results showed that SWE was an effective technique to extract and separate polysaccharides from Sagittaria sagittifolia L., which may be potentially applied in the related polysaccharides extraction. (C) 2019 Elsevier B.V. All rights reserved.
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