Regulating effect of baicalin on IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins in rats with ulcerative colitis

Objective: This study was aimed to explore effect of baicalin on IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins in rats with ulcerative colitis (UC). Methods: Histopathological observation and scores of colon tissue were performed in the UC rat model. IKK/ IKB/NF-kB signaling pathway and apoptosis-related proteins were measured by Western blotting. Results: Baicalin significantly increased the activity of SOD, CAT and GSH-Px in colon tissue of rats with UC, but significantly decreased the content of MDA, IL-1 beta, MPO, PEG2 and TNF-alpha in colon tissue of rats with UC. In the molecular mechanism, baicalin significantly decreased the expression of deaved-caspase3, cleaved-caspase9, Bcl-2/Bax, cyt-c, NF-kB p-65, p-IKK beta/IKK beta and p-IKB alpha/IKB alpha. Baicalin could significantly inhibit p-IKB alpha/IKB alpha content change, but had no significant effect on p-IKK beta/1KK beta. Conclusion: Baicalin may have a regulating effect on IKIVIKB/NF-kB signaling pathway and apoptosis-related proteins in UC rats.