期刊
FISH & SHELLFISH IMMUNOLOGY
卷 92, 期 -, 页码 690-697出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2019.06.060
关键词
Macrophage expressed gene 1; Antiparasitic activity; Antibacterial activity; Epinephelus coioides; Cryptoccuyon irritans
资金
- National Natural Science Foundation of China [41876162]
- Pearl River S and T Nova Program of Guangzhou [201806010181]
- Guangdong MEPP Fund [.2018C001]
- China Modern Agricultural Industry Technology System (The Control of Parasites Infection on Marine Fish) [CARS-47-18]
Macrophage expressed gene 1 (Mpegl) is a molecule that can form pores and destroy the cell membrane of invading pathogens. In this study, we identified two Mpegl isoforms from the orange-spotted grouper (Epinephelus coioides) and named them EcMpegla and EcMpeg1b. Predicted proteins of the two EcMpeg1s contained a signal peptide, a conserved membrane attack complex/perforin (MACPF) domain, a transmembrane segment, and an intracellular region. Sequence alignment demonstrated that two EcMpeg1 proteins share a high sequence identity with that of other teleosts. Tissue distribution analysis showed that EcMpeg1s were expressed in all tissues tested in healthy grouper, with the highest expression in the head kidney and spleen. After infection with the ciliate parasite Cryptocaryon irritans, expression of the two EcMpeg1s was significantly upregulated in the spleen and gills. Furthermore, the recombinant EcMpeg1a showed antiparasitic and antibacterial activity against Gram-negative and-positive bacteria, whereas EcMpeg1b had an inhibitory effect only against Grampositive bacteria. These results indicated that EcMpeg1s play an important role in the host response against invading pathogens.
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