期刊
FEBS LETTERS
卷 594, 期 1, 页码 126-134出版社
WILEY
DOI: 10.1002/1873-3468.13550
关键词
Archaea; homocitrate synthase; lysine biosynthesis; RAM domain
资金
- JSPS KAKENHI [24228001, 17H06168]
- Grants-in-Aid for Scientific Research [17H06168] Funding Source: KAKEN
The hyperthermophilic archaeon, Sulfolobus, synthesizes lysine via the alpha-aminoadipate pathway; however, the gene encoding homocitrate synthase, the enzyme responsible for the first and committed step of the pathway, has not yet been identified. In the present study, we identified saci_1304 as the gene encoding a novel type of homocitrate synthase fused with a Regulation of Amino acid Metabolism (RAM) domain at the C terminus in Sulfolobus acidocaldarius. Enzymatic characterization revealed that Sulfolobus homocitrate synthase was inhibited by lysine; however, the mutant enzyme lacking the RAM domain was insensitive to inhibition by lysine. The present results indicated that the RAM domain is responsible for enzyme inhibition.
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