期刊
EXPERIMENTAL CELL RESEARCH
卷 384, 期 2, 页码 -出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2019.111641
关键词
Mitophagy; Parkin; Ubiquitin-specific protease USP36; Deubiquitinating enzyme; Beclin-1; Autophagy-related protein ATG14L
资金
- Deutsche Forschungsgemeinschaft (DFG) [GE 2844/1-1]
- Faculty of Medicine, Tfibingen [2072-0-0]
- DFG [GRK 2364/1]
- Hertie Foundation
Parkin is an ubiquitin ligase regulating mitochondrial quality control reactions, including the autophagic removal of depolarized mitochondria (mitophagy). Parkin-mediated protein ubiquitinations may be counteracted by deubiquitinating enzymes (DUBS). We conducted a high-content imaging screen of Parkin translocation to depolarized mitochondria after siRNA mediated silencing of each DUB in Parkin overexpressing HeLa cells. Knockdown of the ubiquitin-specific protease USP36 led to delayed Parkin translocation while only slightly disturbing the ubiquitination of mitochondrial proteins, but final autophagic elimination of mitochondria was severely disrupted. The localization of the nucleolar USP36 was not altered during mitophagy. However, the marker for transcriptional active chromatin, histone 2B Lys120 mono-ubiquitination was found reduced in USP36-silenced cells undergoing mitophagy. We observed a reduction of the mRNA and protein levels of Beclin-1 and its associated autophagy-related key regulator ATG14L in USP36 knockdown cells. Importantly, transfection of active ATG14L into USP36-silenced cells significantly restored Parkin-dependent mitophagy. We propose USP36 as regulator for the Parkin-dependent mitophagy at least in part via the Beclin-1-ATG14L pathway.
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