期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 50, 期 1, 页码 119-129出版社
WILEY
DOI: 10.1002/eji.201948129
关键词
CyTOF; immunophenotyping; Sjogren's syndrome; systemic lupus erythematosus; systemic sclerosis
类别
资金
- Biogen Inc. (Cambridge, MA, USA)
Systemic sclerosis (SSc), systemic lupus erythematosus (SLE) and primary Sjogrens syndrome (pSS) are clinically distinct systemic autoimmune diseases (SADs) that share molecular pathways. We quantified the frequency of circulating immune-cells in 169 patients with these SADs and 44 healty controls (HC) using mass-cytometry and assessed the diagnostic value of these results. Alterations in the frequency of immune-cell subsets were present in all SADs compared to HC. Most alterations, including a decrease of CD56(hi) NK-cells in SSc and IgM(+) Bcells in pSS, were disease specific; only a reduced frequency of plasmacytoid dendritic cells was common between all SADs Strikingly, hierarchical clustering of SSc patients identified 4 clusters associated with different clinical phenotypes, and 9 of the 12 cell subset-alterations in SSc were also present during the preclinical-phase of the disease. Additionally, we found a strong association between the use of prednisone and alterations in B-cell subsets. Although differences in immune-cell frequencies between these SADs are apparent, the discriminative value thereof is too low for diagnostic purposes. Within each disease, mass cytometry analyses revealed distinct patterns between endophenotypes. Given the lack of tools enabling early diagnosis of SSc, our results justify further research into the value of cellular phenotyping as a diagnostic aid.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据