Ecological impact of the antibiotic ciprofloxacin on microbial community of aerobic activated sludge

This study investigated the effects and fate of the antibiotic ciprofloxacin (CIP) at environmentally relevant levels (50-500 mu g/L) in activated sludge (AS) microbial communities under aerobic conditions. Exposure to 500 mu g/L of CIP decreased species diversity by about 20% and significantly altered the phylogenetic structure of AS communities compared to those of control communities (no CIP exposure), while there were no significant changes upon exposure to 50 mu g/L of CIP. Analysis of community composition revealed that exposure to 500 mu g/L of CIP significantly reduced the relative abundance ofRhodobacteraceaeandNakamurellaceaeby more than tenfold. These species frequently occur in AS communities across many full-scale wastewater treatment plants and are involved in key ecosystem functions (i.e., organic matter and nitrogen removal). Our analyses showed that 50-500 mu g/L CIP was poorly removed in AS (about 20% removal), implying that the majority of CIP from AS processes may be released with either their effluents or waste sludge. We therefore strongly recommend further research on CIP residuals and/or post-treatment processes (e.g., anaerobic digestion) for waste streams that may cause ecological risks in receiving water bodies.