Mir-142-3p Regulates Inflammatory Response by Contributing to Increased TNF-α in Chronic Rhinosinusitis With Nasal Polyposis

Objective: Previous studies suggested that microRNAs played an important role in the progression of inflammation and remodeling of chronic rhinosinusitis with nasal polyposis. However, the abnormal expression of microRNAs and regulation cytokine expression in nasal polyposis are not clear. Method: The miR-142-3p and tumor necrosis factor alpha (TNF-alpha) expression levels in chronic rhinosinusitis with nasal polyposis were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The miR-142-3p and TNF-alpha levels in human nasal epithelial cells (HNEpC) after stimulation by lipopolysaccharide (LPS) were detected by qRT-PCR. Moreover, HNEpCs were transfected by miR-142-3p mimics or inhibitor or cotransfected with si-TNF-alpha to evaluate the regulation of miR-142-3p on TNF-alpha which affects the production of inflammatory factors. Results: The miR-142-3p and TNF-alpha were significantly higher in nasal mucosa of chronic rhinosinusitis with polyps patients compared to normal human. MiR-142-3p and TNF-alpha expression levels were increased after LPS stimulation in a dose- and time-dependent manner. Knockdown of miR-142-3p in HNEpCs downregulated TNF-alpha expression at both messenger RNA and protein levels. Conclusions: It is indicated that miR-142-3p may participate in the regulation of the body's inflammatory response through the LPS-TLR-TNF-alpha signaling pathway in chronic rhinosinusitis with nasal polyposis.

Automated summary

This study showed that miR-142-3p and TNF-alpha expression levels were significantly elevated in chronic rhinosinusitis with nasal polyps patients, and increased in response to LPS stimulation. Knockdown of miR-142-3p in HNEpCs downregulated TNF-alpha expression at both mRNA and protein levels. It is suggested that miR-142-3p may participate in regulating the body's inflammatory response via the LPS-TLR-TNF-alpha signaling pathway in chronic rhinosinusitis with nasal polyposis.