期刊
EMBO REPORTS
卷 20, 期 10, 页码 -出版社
WILEY
DOI: 10.15252/embr.201947880
关键词
brain development; computer vision; neural stem cells; robotics; single cell manipulation
资金
- Mechanical Engineering department, College of Science and Engineering, MnDRIVE RSAM initiative of the University of Minnesota
- McGovern Institute Neurotechnology (MINT) fund
- National Institutes of Health (NIH) [1R21NS103098-01]
- NSF IGERT training fellowship
- NSF IGERT travel award
- NOMIS Distinguished Scientist Award
- DFG [SFB 655]
- ERC [250197]
- ERA-NET NEURON (MicroKin)
Microinjection into single cells in brain tissue is a powerful technique to study and manipulate neural stem cells. However, such microinjection requires expertise and is a low-throughput process. We developed the Autoinjector, a robot that utilizes images from a microscope to guide a microinjection needle into tissue to deliver femtoliter volumes of liquids into single cells. The Autoinjector enables microinjection of hundreds of cells within a single organotypic slice, resulting in an overall yield that is an order of magnitude greater than manual microinjection. The Autoinjector successfully targets both apical progenitors (APs) and newborn neurons in the embryonic mouse and human fetal telencephalon. We used the Autoinjector to systematically study gap-junctional communication between neural progenitors in the embryonic mouse telencephalon and found that apical contact is a characteristic feature of the cells that are part of a gap junction-coupled cluster. The throughput and versatility of the Autoinjector will render microinjection an accessible high-performance single-cell manipulation technique and will provide a powerful new platform for performing single-cell analyses in tissue for bioengineering and biophysics applications.
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