期刊
DYES AND PIGMENTS
卷 168, 期 -, 页码 77-83出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2019.04.046
关键词
Peroxynitrite; Fluorescence probe; NIR; Large Stokes shift; In vivo imaging
资金
- National Natural Science Foundation of China of China [21672021, 21572018, 21372024, 21232005]
Peroxynitrite (ONOO-) is a kind of reactive oxygen species (ROS) which is associated with pathogenesis of many diseases. A new near-infrared fluorescence probe (DCM-OH) which based on dicyanomethylene-4H-pyrans to detect endogenous ONOO- was designed and synthesized. The two-photon absorption cross sections and large Stokes shift make the probe deeper issue penetration and lower self-absorption. The obtained results demonstrated that probe DCM-OH could sensitively detect ONOO- with a low detection limit (53 nM). What's more, probe DCM-OH exhibited an ultrafast response rate (within 5 s) toward ONOO-, which would be in favor of tracking the highly reactive and short-lived ONOO- in the living systems. Moreover, DCM-OH was successfully employed for imaging endogenous ONOO- in HepG2/RAW 264.7 cells and further applied to visualize oxidative stress in mouse model of inflammation.
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