期刊
CYTOMETRY PART A
卷 95, 期 12, 页码 1226-1230出版社
WILEY
DOI: 10.1002/cyto.a.23880
关键词
antigen-presenting cells; myeloid cells; macrophages; dendritic cells; immunophenotyping; lymph node; mouse
资金
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases
- Foundation for the National Institutes of Health through the Collaboration for AIDS Vaccine Development of the Bill & Melinda Gates Foundation [OPP1147555]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [ZIAAI005027] Funding Source: NIH RePORTER
- Bill and Melinda Gates Foundation [OPP1147555] Funding Source: Bill and Melinda Gates Foundation
This 20-color flow cytometry panel was designed to resolve the cellular heterogeneity of antigen-presenting cells and was optimized for lymph node tissue. Reagents were carefully selected and optimized for identification of B cells (B220), neutrophils (Ly6G), monocytes and macrophages (Ly6C, CD169, F4/80), and dendritic cells (XCR1, CD172a, CD11c, I-A/I-E, CD24, CD64, pDCA-1, CD103, CD11b). Inclusion of additional functional markers involved in cell migration (CCR7), co-stimulation (CD80), and adhesion (ICAM-1) enabled further phenotypic characterization. Finally, this panel has been tested and is compatible with fluorescently labeled antigens such as Alexa Fluor 488 (Ax488) for the study of antigen-bearing cells in vivo. Published 2019. This article is a U.S. Government work and is in the public domain in the USA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据