期刊
CURRENT PHARMACEUTICAL DESIGN
卷 25, 期 45, 页码 4806-4812出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1381612825666190930094019
关键词
MiR-144-3p; FZD4; Steroid-associated osteonecrosis; Mesenchymal stem cells; ONFH; Real-time PCR
资金
- National Natural Science Foundation of China [81501894, 81301052, 81501888]
Background: MicroRNAs have recently been recognized to be engaged in the development of bone diseases. Objective: This study was performed to elucidate the effects of miR-144-3p on proliferation and osteogenesis of mesenchymal stem cells (MSCs) from the patients with steroid-associated osteonecrosis (ONFH) and its related mechanism. Method: The expression level of miR-144-3p in the MSCs from the proximal femur of the patients was examined by Real-time PCR. The cell proliferation ability was assayed by MTT. The differentiation ability of MSCs was assayed by Alizarin Red S (ARS) staining. The interaction between miR-144-3p and frizzled4 (FZD4) was investigated by Real-time PCR, western blot and luciferase reporter assay. Results: ONFH samples had the obviously high expression of miR-144-3p compared to the control. MiR-144-3p had a negative effect on the proliferation and osteogenesis of MSCs. Via targeting FZD4, miR-144-3p decreased beta-catenin nuclear translocation, the transcription of RUNX2 and COLIAI. Over-expression of FZD4 partially reversed miR-144-3p-induced decrease in the proliferation and osteogenesis of MSCs. Conclusion: MiR-144-3p might play an important role in the development of ONFH and might be used as a novel class of therapeutic targets for this disease.
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