期刊
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
卷 21, 期 1, 页码 23-36出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1389201020666190822161337
关键词
Coffea arabica; vitamin C; MCF-7; anticancer; apoptosis; protein expression
Background: Vitamin C (VC) is believed to enhance immunity and is regularly integrated as a supplementary agent during several treatments. Objective: The green (GC) and roasted (RC) coffee (Coffee: arabica) aqueous extracts (0, 125, 250 and 500 mu g/ml) combined with VC (50 mu g/ml) were examined on the cancerous MCF-7 cell line and normal human lymphocytes. Methods: Neutral red uptake assay, comet assay, immtmocytochemical reactivity for protein expression and mRNA expression of apoptosis-related genes were performed. Results: A significant (P< 0.05) concentration-dependent increase of apoptotic features, such as morphological changes, and abundant nuclear condensation, altered the expression of p53 and caspase-3 mRNA, down-regulation of Bcl-2 protein as well as the acidic autophagosomal vacuolization in treated cells. The oxidative stress and DNA single-strand breaks were noticed too. Conclusion: These results suggest that coffee in combination with VC undergoes apoptotic anticancer pathway. This supports the integration of coffee and VC as a valuable candidate for anticancer research and treatments.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据