期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 23, 期 8, 页码 1728-1734出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2015.02.045
关键词
Carbonic anhydrase; Metalloenzymes; Hydratase activity; Antarctic carbonic anhydrase; Cyanobacteria
资金
- Italian National Antarctic Research Project [PNRA 2013/AZ1.02]
A carbonic anhydrase (CA, EC 4.2.1.1) belonging to the gamma-class has been cloned, purified and characterized from the Antarctic cyanobacterium Nostoc commune. The enzyme showed a good catalytic activity for the physiologic reaction (hydration of carbon dioxide to bicarbonate and a proton) with the following kinetic parameters, k(cat) of 9.5 x 10(5) s(-1) and k(cat)/K-M of 8.3 x 10(7) M-1 s(-1), being the gamma-CA with the highest catalytic activity described so far. A range of aromatic/heterocyclic sulfonamides and one sulfamate were investigated as inhibitors of the new enzyme, denominated here NcoCA. The best NcoCA inhibitors were some sulfonylated sulfanilamide derivatives possessing elongated molecules, aminobenzolamide, acetazolamide, benzolamide, dorzolamide, brinzolamide and topiramate, which showed inhibition constants in the range of 40.3-92.3 nM. As 1,5-bisphosphate carboxylase/oxygenase (RubisCO) and gamma-CAs are closely associated in carboxysomes of cyanobacteria for enhancing the affinity of RubisCO for CO2 and the efficiency of photosynthesis, investigation of this new enzyme and its affinity for modulators of its activity may bring new insights in these crucial processes. (C) 2015 Elsevier Ltd. All rights reserved.
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