期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 181, 期 -, 页码 705-713出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2019.06.011
关键词
Alpha mangostin; Fibroin; Crosslinked; Nanoparticles; Controlled release; Cancer
资金
- Thailand Research Fund (TRF) under the Royal Golden Jubilee (RGJ) Ph.D. Grant [PHD/0234/2560 RGJ]
- Naresuan University [R2561B009]
- Naresuan University ASEAN Scholarship
- RGJ Ph.D. Grant
- Boosting Research Potential of Naresuan University Students program, Batch 4
- Naresuan University Scholarship for Oral Presentation
Silk fibroin has been utilized extensively for biomedical purposes, especially the drug delivery systems. This study introduced and characterized three novel alpha-mangostin loaded crosslinked fibroin nanoparticles (FNPs), using EDC or PEI as a crosslinker, for cancer treatment. All three formulas were spherical particles with a mean size of approximately 300 tun. By varying the type and/or amount of the crosslinkers, particle surface charge was controllable from -15 to +30 mV. Crosslinked FNPs exhibited higher drug entrapment efficiency (70%) and drug loading (7%) than non-crosslinked FNP. FT-IR, XRD, and DSC analytical methods confirmed that alpha-mangostin was entrapped in FNPs in molecular dispersion form. Compared to the free alpha-mangostin, the crosslinked FNPs increased the drug's solubility up to threefold. They also showed sustained release characteristics of more than 3 days, and reduced free alpha-mangostin hematotoxicity by 90%. The alpha-mangostin loaded FNPs were physicochemically stable for up to 24 h when dispersed in intravenous diluent and for at least 6 months when preserved as lyophilized powder at 4 degrees C. In terms of anticancer efficacy, on both Caco-2 colorectal and MCF-7 breast adenocarcinoma cell lines, all formulas maintain alpha-mangostin's apoptotic effect while exhibit greater cytotoxicity than the free drug. In conclusion, alpha-mangostin loaded crosslinked FNPs show high potential for cancer chemotherapy.
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