期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 181, 期 -, 页码 935-942出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2019.06.010
关键词
Nanotechnology; Polymeric nanoparticles; Essential oil; Cymbopogon citratus; Box-Behnken design; Cytotoxicity
资金
- Fundacao de Amparo a Pequisa do Estado do Rio de Janeiro (FAPERJ) - Brazil [JCNE 2016, E-26/202,759/2017, E26/202,256/2018]
- (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior CAPES) - Brazil [001]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) - Brazil [302345/2017-5]
This study aimed to optimize Cymbopogon citratus essential oil loaded into PLGA-nanoparticles by investigating the effect of processing variables (sonication time, ultrasound power, and essential oil/polymer ratio) on encapsulation efficiency and particle mean hydrodynamic diameter using Box-Behnken design. Nanoparticles were prepared by an emulsification/solvent diffusion method and physicochemically characterized by FTIR, DSC and TGA/DTA. Cytotoxicity was evaluated in human HaCat keratinocytes by WST-1 and LDH assays. The optimized formulation had a hydrodynamic mean diameter of 277 nm, a polydispersity index of 0.18, a Zeta potential of -16 mV and an encapsulation efficiency of 73%. Nanoparticle characterization showed that only citral was incorporated in nanocarriers, with some amount adsorbed on their surface, and highlighted the potential in increasing the oil thermal stability. The drug release profile demonstrated a biphasic pattern with a substantial sustained release depending on diffusion from the polymeric matrix. Toxicity effects on cell viability of pure essential oil at low concentrations were significantly eliminated when encapsulated. Results revealed the ability of PLGA-nanoparticles to improve essential oil physicochemical characteristics, by controlling release and reducing toxicity, suggesting their potential use in pharmaceutical preparations.
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