Structural elucidation and bioactivities of a novel arabinogalactan from Coreopsis tinctoria

Coreopsis tinctoria is being widely cultivated in Xinjiang of China, whose consumption is known to prevent diabetes and neurodegenerative diseases. To elucidate the bioactive ingredients responsible for these benefits, the alkaline soluble crude polysaccharide (CTB) was isolated from C. tinctoria. In vitro experiments showed that the inhibition of alpha-amylase and alpha-glucosidase by CTB was 13407-fold and 906-fold higher than that by positive control, respectively. Then, a novel arabinogalactan, CTBP-1, was isolated and purified from CTB. Structural analysis showed that CTBP-1 possessed a 1,6-linked beta-D-Galp and 1,5-linked alpha-L-Araf backbone with branches substituted at the C-3 position of the 1,6-linked beta-D-Galp, and the side chains included 1,5-linked alpha-L-Araf, T-linked beta-D-Galp and T-linked alpha-L-Aral. The inhibitory effects of CTBP-1 on alpha-amylase and alpha-glucosidase were 2.7 and 17.9 times that of acarbose, respectively. CTBP-1 could avoid indigestion and similar side effects. In addition, CTBP-1 remarkably inhibited the release of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. In summary, CTBP-1 is a novel arabinogalactan with great potential as a treatment for type 2 diabetes and Alzheimer's disease.