期刊
BRITISH POULTRY SCIENCE
卷 60, 期 6, 页码 798-801出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/00071668.2019.1650163
关键词
Antibiotic resistance; chicken meat; E; coli; genes encoding aminoglycoside-modifying enzymes; virulence genes
1. The aim of the experiment was to determine the occurrence of genes encoding aminoglycoside-modifying enzymes (AMEs) in Escherichia coli isolates recovered from chicken meat. 2. Antibiotic sensitivity was tested using the disc diffusion test. AMEs and virulence profile were determined by PCR/sequencing. 3. Out of 195 meat samples collected, 185 (95%) isolates were identified as E. coli. Disc diffusion showed a resistance value of 22% (n = 42) for at least one of the antibiotic aminoglycosides (AGs) tested (tobramycin, gentamycin, amikacin and kanamycin). PCR screening showed the presence of three classes of AMEs, namely, aac(3)-II (12%), aac(6MODIFIER LETTER PRIME)-Ib (7%) and aac(2MODIFIER LETTER PRIME)-Ia (5%). Eight of the 42 isolates were positive for the stx(1) and sxt(2) genes and were defined as Shiga toxin-producing E coli., while the eae gene was positive in one strain. Among the 42 isolates, group A was the predominant phylogenetic identified (76%), followed by group D (21%). One isolate belonged to subgroup B2(3). 4. The results suggested that chicken meat could be an important reservoir of AMEs, and pose a potential risk by dissemination of resistance to humans through the food chain.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据