期刊
BRITISH JOURNAL OF NUTRITION
卷 122, 期 10, 页码 1081-1090出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114519001910
关键词
Selenoprotein; Heat stress; IPEC-J2 cells; Sodium selenite; Selenomethionine
资金
- National Natural Science Foundation of China [31772643, 31272468]
- Foundation of Educational Department of Sichuan [17ZA0318]
- Special Research Funding for Discipline Construction in Sichuan Agricultural University [03570126]
The present study compared the protective effect of sodium selenite (SS) and selenomethionine (SeMet) on heat stress (HS)-invoked porcine IPEC-J2 cellular damage and integrate potential roles of corresponding selenoprotein. Cells were cultured at 37 degrees C until 80 % confluence and then subjected to four different conditions for 24 h: at 37 degrees C (control), 41 center dot 5 degrees C (HS), 41 center dot 5 degrees C supplied with 0 center dot 42 mu mol Se/L SS (SS), or SeMet (SeMet). HS significantly decreased cell viability, up-regulated mRNA and protein levels of heat shock protein 70 (HSP70) and down-regulated mRNA and protein levels of tight junction-related proteins (claudin-1 (CLDN-1) and zonula occludens-1 (ZO-1)). HS-induced cell injury was associated with the up-regulation (P < 0 center dot 05) of six inflammation-related genes and fourteen selenoprotein encoding genes and down-regulation (P < 0 center dot 05) of two inflammation-related genes and five selenoprotein encoding genes. Compared with the HS group, SS and SeMet supplementation resulted in an increase (P < 0 center dot 05) in cell viability, decreased (P < 0 center dot 05) mRNA expression of HSP70 and six inflammation-related genes and rescue (P < 0 center dot 05) of mRNA and protein levels of CLDN-1 and ZO-1. SS and SeMet supplementation changes the expressions of nineteen selenoprotein encoding genes in cells affected by HS. Both Se supplementation significantly recovered the protein level of glutathione peroxidase-1 and increased selenoprotein P in the IPEC-J2 cells under HS, respectively. In summary, Se supplementation alleviated the negative impact of HS on IPEC-J2 cells, and their cellular protective effect was associated with regulation expression of selenoproteins, and SeMet exhibited a better protective effect.
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