期刊
BREAST CANCER RESEARCH AND TREATMENT
卷 178, 期 2, 页码 251-261出版社
SPRINGER
DOI: 10.1007/s10549-019-05374-x
关键词
Breast carcinoma; Stem cell; Mutation; Droplet digital polymerase chain reaction; Metastasis
类别
资金
- 2013 Conquer Cancer Foundation of ASCO Long-term International Fellowship (LIFe) in Breast Cancer
- Breast Cancer Research Foundation
Purpose Limited knowledge exists on the detection of breast cancer stem cell (BCSC)-related mutations in circulating free DNA (cfDNA) from patients with advanced cancers. Identification of new cancer biomarkers may allow for earlier detection of disease progression and treatment strategy modifications. Methods We conducted a prospective study to determine the feasibility and prognostic utility of droplet digital polymerase chain reaction (ddPCR)-based BCSC gene mutation analysis of cfDNA in patients with breast cancer. Results Detection of quantitative BCSC gene mutation in cfDNA by ddPCR mirrors disease progression and thus may represent a valuable and cost-effective measure of tumor burden. We have previously shown that hematological and neurological expressed 1-like (HN1L), ribosomal protein L39 (RPL39), and myeloid leukemia factor 2 (MLF2) are novel targets for BCSC self-renewal, and targeting these genetic alterations could be useful for personalized genomic-based therapy. Conclusion BCSC mutation detection in cfDNA may have important implications for diagnosis, prognosis, and serial monitoring.
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