期刊
BRAIN RESEARCH
卷 1717, 期 -, 页码 136-146出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2019.04.021
关键词
POMC; Melanocortin System; Food intake; Hypothalamus; Leptin
资金
- Sao Paulo Research Foundation (FAPESP) [17/02983-2, 17/16473-6]
- National Institute for Translational Neuroscience
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [17/16473-6] Funding Source: FAPESP
The central melanocortin system is composed of neurons that express either the proopiomelanocortin (POMC) or the agouti-related protein (AgRP). POMC is cleaved in bioactive peptides, including the alpha-melanocyte-stimulating hormone (alpha-MSH). alpha-MSH activates the melanocortin-4 receptor (MC4R) inducing satiety, whereas AgRP acts as an inverse agonist of MC4R. However, only limited information is available regarding possible area-specific differences in the interaction between alpha-MSH and AgRP terminals on MC4R-expressing cells. Therefore, the objective of the present study was to compare the distribution pattern of alpha-MSH and AgRP terminals on the perikarya of MC4R-expressing neurons. We performed a triple-label immunofluorescence reaction in brain series of MC4R-reporter mice to visualize MC4R-expressing neurons together with AgRP and a-MSH terminals. POMC and AgRP neurons project to areas that contain MC4R-expressing cells, although several brain nuclei exhibit AgRP and alpha-MSH terminals, but they do no express MC4R, while other brain areas contain MC4R-expressing cells and receive no apparent innervation of AgRP and POMC neurons. AgRP terminals make more presumptive appositions than a-MSH on the soma of MC4R-expressing neurons of the medial preoptic area and paraventricular nucleus of the hypothalamus (Pa). Additionally, a higher percentage of MC4R cells receive at least one presumptive apposition from AgRP terminals in the median preoptic nucleus and Pa, compared to alpha-MSH appositions. Thus, our study revealed area-specific differences in the interaction between alpha-MSH and AgRP terminals and the soma of MC4R-expressing neurons. These findings provide new insights about the relationship between first- and second-order neurons of the central melanocortin system.
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